Recombinant human STAT1-alpha expressed in E. coli.
Conjugate
Unconjugated
Form
Liquid
Purification
purified
Storage buffer
PBS, pH 7.2, with 1% BSA
Contains
0.1% sodium azide
Storage Conditions
-20°C
Tested Applications
Dilution *
ChIP assay (ChIP)
5µl
Flow Cytometry (Flow)
3-5 µg/million cells
Immunocytochemistry (ICC)
1µg/mL
Immunofluorescence (IF)
1µg/mL
Immunohistochemistry (Paraffin) (IHC (P))
1:10-1:100
Immunoprecipitation (IP)
0.5-1 µg/ml
Western Blot (WB)
0.5-1 µg/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Background/Target Information
STAT1 (also known as signal transducers and activators of transcription) is a member of the STAT family of transcription factors. STAT proteins undergo phosphorylation in response to cytokines and growth factors and are translocated to the nucleus. STAT1 can be activated by interferon-alpha, interferon-gamma, EGF, PDGF and IL6. The protein is known to regulate several genes which are involved in cell growth, apoptosis, immune responses, and lipid metabolism. The STAT1 gene is located on chromosome 2.
原厂资料:
注意事项:
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.