A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS), of human Bcl-X protein. Thisamino acid sequence is shared by human and murine Bcl-X protein.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.2 mg/ml
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
0.09% sodium azide
Storage Conditions
4° C
Tested Applications
Dilution *
Flow Cytometry (Flow)
Assay Dependent
Immunocytochemistry (ICC)
1µg/mL
Immunofluorescence (IF)
1µg/mL
Immunohistochemistry (Paraffin) (IHC (P))
Assay-Dependent
Western Blot (WB)
1:500-1:2000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody reacts with both Bcl-XS (short) and Bcl-XL (long) proteins. The immunogenic sequence (aa 3-14) is conserved in human and mice.
Staining of formalin-fixed paraffin-embedded tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 20 min followed by cooling at room temperature for 20 min. A recommended positive control is Hodgkin's lymphoma.
Background/Target Information
Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein, and Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain that is well-conserved among members of the Bcl-2 family. This family of proteins is an important regulator of apoptosis. Bcl xL forms heterodimers with BAX, BAK, and Bcl2, and its overexpression in tumor cells confers resistance against chemotherapeutic drugs. Bcl xL is phosphorylated on many sites including serine 62, a critical site for Bcl xL response to microtubule-damaging drugs such as taxol and vinblastine. Phosphorylation of serine 62 is thought to be mediated by Jun N-terminal stress kinase (JNK) signaling - negatively regulates the anti-apoptotic function of Bcl xL and controls the growth of neoplastic cells.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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