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ReadiLink™ iFluor™ 780 Protein Labeling Kit *Microscale Optimized for Labeling 100 μg An

 
包装: 1 kit
运保温度: Freeze (<-15 °C) Avoid light
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其它组分:

iFluor™ 780 SE【包装:1 vial,,运保温度:-20 °C】
Reaction Buffer【包装:1 vial (200 μL),,运保温度:Do not freeze】
DMSO【包装:1 vial (100 μL),,运保温度:-20 °C】
Spin Column【包装:2 columns,,运保温度:Do not freeze】
Washing Tube (2 mL)【包装:2 tubes,,运保温度:Do not freeze】
Collecting Tube (1.5 mL)【包装:2 tubes,,运保温度:Do not freeze】


描述:

Although FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence. Protein conjugates prepared with iFluor™ 488 dyes (Ex/Em=497 nm/525 nm) are far superior compared to conjugates of fluorescein derivatives such as FITC. iFluor™ 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Additionally, the fluorescence of iFluor™ 488 is not affected by pH (4-10). This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9. iFluor™ 488 SE dye used in the kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. These labeling kits have all the essential components with an optimized labeling protocol. This iFluor™ 488 labeling kit is optimized for labeling ~100 µg antibody.


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