AAR43 specifically recognises an epitope within the C-terminal (CT) region of endothelin B receptor (ET-B), a widely distributed transmembrane protein and member of the G-protein coupled receptor 1 family, which acts as a non-specific receptor for endothelin 1, 2, and 3, and mediates its action by G protein association and activation of a phosphatidylinositol-calcium second messenger system.
AAR43 recognizes epitopes on the cytoplasmic side of the receptor and therefore the cell membrane must be permeable to the antibody. AAR43 does not block the binding of endothelins.
AAR43 is reported as suitable for use in immunocytochemistry on acetone fixed cells.
Species Cross-Reactivity
Target Species
Cross Reactivity
Human
Yes
Mouse
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunofluorescence
10 - 20ug/ml
Immunohistology - Frozen
Immunohistology - Paraffin
10 - 20ug/ml
Immunoprecipitation
20 - 40ug/ml
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to rat ET-B (CT) was raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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