AAM69 recognises mouse CCR10 (C-C chemokine receptor type 10), otherwise known as GPR2, (G-protein coupled receptor 2), a transmembrane glycoprotein and member of the G-protein coupled receptor 1 family, highly expressed in the small intestine, colon, lymph nodes and Peyer patches, which acts as a receptor for the chemokines CCL27 (SCYA27/CTACK) and CCL28 (SCYA28).
AAM69 is reported as suitable for use in immunocytochemistry. AAM69 shows minimal cross-reactivity with human CCR10.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/100000 -
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
1/250 -
Immunoprecipitation
Western Blotting
1/1000 -
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106cells in 100ul.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to mouse CCR10 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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