9129-7995 recognises TGF-alpha (transforming growth factor alpha) from human, rat and bovine tissues and fluids. TGF-alpha is a 50 amino acid EGF-related polypeptide expressed in a range of tissues, including skin, pancreas, liver, duodenum, salivary glands, urinary and respiratory systems, and the developing and mature central nervous system (CNS).
TGF-alpha is produced by monocytes, keratinocytes and neuronal cells and stimulates the proliferation of a range of epidermal and epithelial cells, through binding to the epidermal growth factor receptor (EGFR). TGF-alpha is mitogenic for multipotent neural progenitors and is involved in astrogliosis.
This antibody does not react with EGF or TGF-beta.
Species Cross-Reactivity
Target Species
Cross Reactivity
Bovine
Yes
Rat
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
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