Recombinant protein corresponding to amino acids 20–170 of human NCAM
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/ml
Purification
Protein A
Storage buffer
PBS
Preservative
0.09% sodium azide
Storage Conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Tested Applications
Dilution *
Flow Cytometry (Flow)
1 ug/ml X 10^6 cells
Immunocytochemistry (ICC)
1 ug/ml
Immunofluorescence (IF)
1 ug/ml
Western Blot (WB)
0.5-2 ug/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
This antibody is predicted to react with bovine, canine, chimpanzee, feline, non-human primate and rabbit based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Background/Target Information
The neural cell adhesion molecule (NCAM) is a member of the Ig superfamily, and is involved in mediating homophilic binding. A number of different isoforms of NCAM exist. The isoforms are differentially expressed depending upon tissue type, and stage of development. In addition to playing a role in cell-cell adhesion, NCAM is also involved in development, neurite outgrowth, synaptic plasticity, learning, and memory.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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