Recombinant protein corresponding to amino acids 215-422 of human MDM2.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/ml
Purification
Protein A
Storage buffer
PBS
Preservative
0.09% sodium azide
Storage Conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Tested Applications
Dilution *
Immunocytochemistry (ICC)
1-3 ug/ml
Immunofluorescence (IF)
1-3 ug/ml
Western Blot (WB)
0.1-1 ug/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
70-0555 has been successfully used in immunofluorescence and western blot. In western blot applications, 70-0555 recognizes the ~55kD and ~75kD isoforms of MDM2.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Background/Target Information
MDM2 also known as murine double minute 2, is found to interact with p53; a tumor suppressor protein, and has been shown to negatively regulate its function. MDM2 binds and inhibits transactivation role played by p53 and overexpression of MDM2 can result in the inactivation of p53 and in turn decrease its tumor suppressor function. Another process by which MDM2 can inactivate p53 is by degrading p53 as the protein also possesses E3 ubiquitin ligase activity. MDM2 also plays various important roles in apoptosis and cell cycle. MDM2 is located on chromosome 12 on the q arm.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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