A recombinant protein corresponding to amino acids 1-187 of Q13114.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/ml
Purification
Protein A
Storage buffer
PBS
Preservative
0.09% sodium azide
Storage Conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Tested Applications
Dilution *
Immunocytochemistry (ICC)
1:100-1:1000
Immunofluorescence (IF)
1:100-1:1000
Immunohistochemistry (Paraffin) (IHC (P))
1:10-1:100
Western Blot (WB)
0.5-1µg/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
This antibody is predicted to react with chimpanzee, Rhesus monkey, mouse, rat, porcine, canine, bovine and equine based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Background/Target Information
TRAF3, also known as TNF receptor-associated factor 3, is an adapter protein and signal transducer that links multiple members of the tumor necrosis receptor family to different signaling pathways. This is done by association with the receptor cytoplasmic domain and various kinases. It also regulates innate immune system by modulating signaling via Toll-like receptors. Overexpression of TRAF3 does not induce activation of the commonly known TRAF-inducible signaling pathways, NF- kappaB and JNK. In contrast, it acts as a negative regulator of the NF- kappaB pathway. Furthermore, TRAF3 is a positive regulator of type I IFN production. These results indicate that TRAF3 is a critical regulator of both innate and adaptive immune responses. In addition to being a major modulator of immune responses, TRAF3 regulates B cell homeostasis in secondary lymphoid organs.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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