5980-1410 recognises MMP14, also known as MT1-MMP, a metalloproteinase involved in the cleavage of type 1 collagen fibres at the cell surface, requiring homodimerisation of MMP-14 via the hemopexin domain. MMP14 is the only membrane anchored collagenase. Besides cleaving collagens types I, II and III, MMP14 also degrades a range of extracellular macromolecules including aggrecan, vitronectin, fibronectin and laminins 1 and 5.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Western Blotting
1/1000 - 1/4000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Western Blotting
This product is suitable for the detection of 30 - 100 ng of MMP-14 using ECL detection.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
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