5980-0311 is specific for Human MMP-3 and does not react with MMP 1, 2, 9 or fibronectin.
MMP-3 is a proteinase that degrades fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Immunohistology - Frozen
Immunohistology - Paraffin
1/10 - 1/100
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Immunohistology
Formalin fixation is recommended for paraffin embedded sections. Carnoys or 70% ethanol may also be suitable.
Western Blotting
Native Human MMP3 (5980-0357) is suitable for use with 5980-0311 in a western blot.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.