5605-1000G recognises human Lactoferrin (LF), also known as Lactotransferrin (LTF), a 78.1kDa secreted protein belonging to the transferrin family of proteins.
Lactoferrin is a multifunctional protein with microbial activity. It inhibits the ability of bacteria and viruses to attach to cell membranes, additionally it acts as an antifungal agent. Research has indicated that lactoferrin may also have a role in inhibiting the formation of protease resistant prion proteins.
Lacoferrin occurs in milk and mucosal secretions including tears and saliva. It has also been detected in a range of cell types including brush border cells, PMN, monocytes, macrophages and activated lymphocytes. Highest concentrations are found in human colostrum.
5605-1000G is reported to work well in formalin fixed paraffin embedded sections of human and canine mammary glands but does not stain canine neutrophils.
Species Cross-Reactivity
Target Species
Cross Reactivity
Dog
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/200 - 1/2000
Immunohistology - Frozen
Immunohistology - Paraffin(1)
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
