A synthetic 11 amino acid peptide derived from the C-terminus of the mouse HDAC2 protein.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.25 mg/ml
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Preservative
0.1% sodium azide
Storage Conditions
-20°C
Tested Applications
Dilution *
ChIP assay (ChIP)
Assay Dependent
ELISA (ELISA)
0.1-1.0 ug/ml
Flow Cytometry (Flow)
3-5 µg/10^6 cells
Immunocytochemistry (ICC)
2-3 µg/mL
Immunofluorescence (IF)
2-3 µg/mL
Immunohistochemistry (Paraffin) (IHC (P))
1:20
Immunoprecipitation (IP)
5 ug
Western Blot (WB)
1-3 µg/mL
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly concerved from yeast to human.
原厂资料:
注意事项:
For Research Use Only. Not for use in diagnostic procedures.