Recombinant protein derived from the C-terminus of human HIF-1-alpha protein
Conjugate
Unconjugated
Form
Frozen
Concentration
0.5 mg/ml
Purification
Protein A
Storage buffer
PBS, pH 7.4
Preservative
0.1% sodium azide
Storage Conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Tested Applications
Dilution *
Western Blot (WB)
2 ug/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Background/Target Information
HIF-1 (hypoxia-inducible factor-1) is a heterodimer consisting of an alpha and a beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF-1 is a key component of a widely operative transcriptional response activated by hypoxia, cobaltous ions, and iron chelation. HIF-1 activates transcription of hypoxia-inducible genes, including those encoding: erythropoietin, vascular endothelial growth (VEGF), heme oxygenase-1, inducible nitric oxide synthase, and the glycolytic enzymes aldolase A, enolase 1, lactate dehydrogenase A, phosphofructokinase I, and phosphoglycerate kinase 1. The C-terminal of HIF-1 alpha binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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