Recombinant protein derived from the C-terminus region of human GAPDH protein
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/ml
Purification
Protein A
Storage buffer
PBS, pH 7.4
Preservative
0.1% sodium azide
Storage Conditions
-20°C
Tested Applications
Dilution *
Flow Cytometry (Flow)
3-5 ug x 10^6 cells
Immunocytochemistry (ICC)
1-2 ug/ml
Immunofluorescence (IF)
1-2 ug/ml
Immunohistochemistry (Paraffin) (IHC (P))
1:50-1:200
Immunoprecipitation (IP)
Assay Dependent
Western Blot (WB)
1-2 ug/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Background/Target Information
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is an enzyme involved in glycolysis. In addition to this role, it is involved in other cellular processes ranging from membrane fusion, neuronal apoptosis to cancer. GAPDH is expressed at high levels in most tissues and is therefore useful as protein loading control in western blot analysis. Additionally, the antigen is conserved in most eukaryotes.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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