Apolipoprotein E (apoE) is a 34 kD heparin binding glycoprotein involved in lipid transport and lipoprotein metabolism. It is present in plasma lipoprotein particles such as chylomicrons, VLDL and HDL. ApoE is also present in large amounts at sites of neurological damage and appears to be involved in recycling of apoptotic remnants and of amyloidal aggregates. ApoE is mainly produced in the liver and by macrophages, but also by other cell types such as smooth muscle cells or neuronal cells. ApoE exists in three isoforms, apoE2, apoE3 and apoE4. Of these, apoE4 is associated with Alzheimer’s disease. Normal concentration of apoE in plasma is approximately 30-40 µg/ml.
Mabtech’s apoE ELISA detects the three apoE isoforms; apoE2, apoE3 and apoE4
All kits include a recombinant apoE3 standard. The test is suitable for EDTA-plasma and citrate-plasma. Heparin plasma can be used, but consideration has to be given to heparin elution of cell surface bound apoE, resulting in higher levels of measured protein.
Mabtech’s enzyme-linked immunosorbent assay, ELISA PRO, is a complete kit for the quantification of apolipoprotein E in biological fluids such as serum, plasma and cell culture supernatants. The assay utilizes ELISA strip plates precoated with a capture monoclonal antibody (mAb), a biotinylated mAb for detection, streptavidin-horseradish peroxidase (SA-HRP), TMB substrate, standard, buffers, diluents, stop solution and adhesive plate covers.
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