Transforming Growth Factor β1 (TGF-β1) is secreted in a latent, biologically inactive form, non-covalently linked with a Latency Associated Protein (LAP). The monoclonal antibodies MT593 and MT517-biotin bind to the LAP entity of human latent TGF-β1. This enables direct quantification of latent TGF-β1 in ELISA with no need of sample pre-treatment. The ELISA does not detect human latent TGF-β2 or -β3 or bovine latent TGF-β1.
Cross-reactivity has been assessed and confirmed with certain non-human primate species.
Mabtech’s enzyme-linked immunosorbent assay, ELISA PRO, is a complete kit for the quantification of latent TGF-β1 in biological fluids such as plasma and cell culture supernatants. The assay utilizes ELISA strip plates precoated with a capture monoclonal antibody (mAb), a biotinylated mAb for detection, streptavidin-horseradish peroxidase (SA-HRP), TMB substrate, standard, buffers, diluents, stop solution and adhesive plate covers.
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