Synthetic peptide derived from the C-terminal sequence of the human connexin 32 protein, downstream from the sequence identified by another Invitrogen Connexin 32 antibody, Cat. No. 71-0600.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.25 mg/ml
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Preservative
0.1% sodium azide
Storage Conditions
-20°C
Tested Applications
Dilution *
Immunohistochemistry (IHC)
1-2 µg/ml
Immunohistochemistry (Frozen) (IHC (F))
Assay Dependent
Immunohistochemistry (Paraffin) (IHC (P))
1:10-1:100
Western Blot (WB)
Assay Dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
34-5700 was used in the immunofluorescence analysis to successfully detect Connexin 32 in gap junctions from mouse liver samples.
Background/Target Information
This gene encodes a member of the gap junction protein family. The gap junction proteins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Mutations in this gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
原厂资料:
注意事项:
For Research Use Only. Not for use in diagnostic procedures.