Synthetic peptide derived from the n-terminal region of human MDM2
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/ml
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 1mg/ml BSA, 30% glycerol
Preservative
0.05% sodium azide
Storage Conditions
-20° C, Avoid Freeze/Thaw Cycles
Tested Applications
Dilution *
Immunocytochemistry (ICC)
1-2 ug/ml
Immunofluorescence (IF)
1-2 ug/ml
Immunohistochemistry (IHC)
Assay Dependent
Immunoprecipitation (IP)
Assay Dependent
Western Blot (WB)
2-3 ug/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody recognizes the ~90 kDa (apparent MW) MDM2 protein. Also recognizes isoforms at ~57 and ~74/76 kDa.
Background/Target Information
MDM2 is a target of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of MDM2 can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
