Interferon-γ (IFN-γ) is produced mainly by activated T cells and NK cells. It is a proinflammatory cytokine that activates macrophages and endothelial cells, but it also regulates immune responses by effecting APC and T and B cells. Production of IFN-γ by helper T cells as well as cytotoxic T cells is a hallmark of the TH1-type phenotype, thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.
The ELISpot kits mentioned below are suitable for the detection of IFN-γ in cow and sheep.
ELISpot PLUS kit for the detection of bovine IFN-γ producing cells. This complete kit contains ELISpot plates with PVDF membrane, capture mAb, biotinylated detection mAb, Streptavidin-ALP and BCIP/NBT substrate.
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