Interleukin-4 (IL-4) is produced by activated T cells, especially differentiated CD4+ TH2-type cells, and stimulated mast cells and basophils. The most important effects of IL-4 are the regulation of helper T cell differentiation to the TH2 type and the regulation of IgE and IgG4 production by B cells.
The ELISpot kits mentioned below are suitable for the detection of IL-4 in cow and sheep.
ELISpot PLUS kit for the detection of bovine-IL4. This complete kit contains ELISpot plates with PVDF membrane, capture mAb, biotinylated detection mAb, Streptavidin-ALP and BCIP/NBT substrate.