2407-1658 reacts with 34 kD cdc2 protein, no reaction was seen against other related cyclin dependent kinases. p34cdc2 complexes with cyclin to form maturation promoting factorThe active form of the enzyme functions by phosphorylating a number of proteins, this is coupled with cell entry into the M-phase of the cell cycle. p34cdc2 must be associated with normal cyclin for the M phase to be completed. Association with deletion mutants of cyclin halts the M phase prior to completion.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Yes
Rat
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/500 - 1/1000
Immunohistology - Frozen
1/100 - 1/500
Western Blotting
1/500 - 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
2407-1658 was prepared from monospecific antiserum by delipidation and defibrination
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