202008 reacts with the heavy chain of human IgM. This antibody has been cross-absorbed to reduce cross-reactivity with human IgG and IgA, but may react with IgM from other species.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/5,000 - 1/20,000
Flow Cytometry
Neat - 1/10
Immunoblotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106cells in 100ul.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from the date of despatch.
Antiserum Preparation
Antiserum to human IgM was raised by repeated immunisation of goats with human IgM paraproteins. The preparation was purified by affinity chromatography on human IgM covalently linked to Sepharose-4B.
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