Description: This 5C4 monoclonal antibody reacts with human Nef-associated factor-1 (Naf-1). This protein is also designated as A20-binding inhibitor of NFkB activation (ABIN-1) and TNFAIP3-interacting protein 1 (TNIP1). Naf-1 is predicted to consist of four leucine zippers and four coiled-coil domains. This protein exists as two isoforms, Naf1α and Naf1β, which are 72-kDa and 68-kDa, respectively, and is expressed in a variety of tissues, with greatest expression in peripheral blood lymphocytes, spleen, and skeletal muscle. Naf1 has also been detected in the Jurkat, Molt-4, and HL60 cell lines.
Naf-1 has been shown to play several biological roles. Naf-1 was originally identified associated with the Nef protein of HIV, and was found to enhance CD4 cell surface expression. Naf-1 has also been demonstrated to inhibit TNF-α- and IL-1-mediated NFkB-dependent gene expression by competing with IKK-γ. Finally, this adaptor protein has been found to associate constitutively with P-selectin glycoprotein ligand 1 (PSGL-1) in human leukocytes. Upon P-selectin binding to PSGL-1, Naf1 is phosphorylated at Tyr552 by Src family kinases, leading to PI3K recruitment and integrin activation.
This antibody recognizes both the unphosphorylated and phosphorylated forms of Naf1α and Naf1β.
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