The mouse Anti-Gr-1 antibody reacts with Ly-6G, a 21-25 kDa, GPI-anchored cell surface protein, previously defined as the granulocyte-differentiation antigen-1 (Gr-1).1The antibody has been shown to cross-react weakly with Ly-6C-transfected EL-4J cells.2Cross-reactivity of Anti-Gr-1 with Ly-6C was not detected on hematopoietic cells that express Ly-6C and are negative for Ly-6G.3
Gr-1 is expressed on mature granulocytes in bone marrow and peripheral tissues. The Anti-Gr-1 antibody also stains monocytes transiently during their differentiation in bone marrow and at low levels plasmacytoid dendritic cells in lymphoid tissues.
When using formaldehyde-fixed cells that express Gr-1 at high levels, for example, granulocytes, the dilution is 1:50 for up to 10⁷ cells/100 µL of buffer for labeling of cells and analyzing by flow cytometry.
原厂资料：
The mouse Anti-Gr-1 antibody reacts with Ly-6G, a 21-25 kDa, GPI-anchored cell surface protein, previously defined as the granulocyte-differentiation antigen-1 (Gr-1).1The antibody has been shown to cross-react weakly with Ly-6C-transfected EL-4J cells.2Cross-reactivity of Anti-Gr-1 with Ly-6C was not detected on hematopoietic cells that express Ly-6C and are negative for Ly-6G.3
Gr-1 is expressed on mature granulocytes in bone marrow and peripheral tissues. The Anti-Gr-1 antibody also stains monocytes transiently during their differentiation in bone marrow and at low levels plasmacytoid dendritic cells in lymphoid tissues.
When using formaldehyde-fixed cells that express Gr-1 at high levels, for example, granulocytes, the dilution is 1:50 for up to 10⁷ cells/100 µL of buffer for labeling of cells and analyzing by flow cytometry.
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