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DMEM 5L, BAG

 
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运保温度: 2-8° C
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描述:

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:

With Without
• High Glucose • Sodium Pyruvate
• GlutaMAX™  
• Phenol Red  



The complete formulation is available.

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g ⁄ L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. Gibco® DMEM with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

Product Intended Use
This Gibco® DMEM formulation has received Pre-market 510k clearance as a Class II medical device for human ex-vivo tissue and cell culture processing applications from the FDA. Customers using Gibco® DMEM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference our Type II Drug Master File (DMF).

cGMP Manufacturing and Quality System
Gibco® DMEM is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers an identical Gibco® DMEM product made in our Scotland facility (61965-026). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g ⁄ L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.


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