0280-1305 is specific for Aldosterone, a hormone produced by the adrenal glands that helps regulate blood pressure through increasing both the release of potassium and the uptake of water and sodium by the kidneys.
0280-1305 shows <1% cross-reactivity with Androstenedione, Corticosterone, DHEA or Desoxycorticosterone, as determined using a competitive ELISA with 400ng of aldosterone-3-BSA per well. This antibody has been cross-absorbed with BSA.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Immunohistology - Frozen
1/25 - 1/200
Immunohistology - Paraffin
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
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