0100-0084 recognizes calenxin, a calcium-binding protein that interacts with newly sythesized glycoproteins in the endoplasmic reticulum.
Target Species
Dog
Species Cross-Reactivity
Target Species
Cross Reactivity
Bovine
Yes
Guinea Pig
Yes
Hamster
Yes
Human
Yes
Monkey
Yes
Mouse
Yes
Pig
Yes
Rabbit
Yes
Rat
Yes
Sheep
Yes
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
50% Glycerol
Immunogen
Synthetic peptide corresponding to the amino acids 50 - 68 of canine calnexin conjugated to KLH.
Buffer Solution
Phosphate buffered saline
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Immunohistology - Frozen
1/100 - 1/400
Immunoprecipitation
1/50 - 1/200
Western Blotting
1/1000 -
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Western Blotting
This antibody detects a band of approximately 98 kDa in 3T3 and HeLa cell lysates under denaturing and reducing conditions.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.